In Vitro Methods in Aquatic EcotoxicologySpringer Science & Business Media, 2003 M08 14 - 472 páginas This book, the first of its kind in this subject area, will focus on in vitro approaches to the study of the toxicology of polluting agents (including heavy metals, radionuclides, micro-organics, estrogenic compounds, and complex mixtures) in the aquatic environment. The importance of in vitro methods is that they allow standardised techniques to be developed and validated for substance and species specific experiments in a controlled way. Also, they allow mechanistic studies without the problems of individual variation between animals and environmental stress. |
Contenido
INTRODUCTION BASIC REQUIREMENTS FOR IN VITRO APPLICATIONS IN AQUATIC ECOTOXICOLOGY | 1 |
CULTURE TECHNIQUES | 7 |
BASIC METHODS AND MEDIA FOR EUKARYOTIC CELLS IN VITRO | 9 |
21 ANIMALS | 10 |
23 PROCEDURES | 11 |
24 CULTURE MEDIA | 15 |
25 CONCLUSIONS | 16 |
DEVELOPMENT OF PRIMARY TISSUE TECHNIQUES FOR USE IN ECOTOXICOLOGY SOME CONSIDERATIONS NECESSARY PRIOR TO ... | 17 |
IN VITRO BIOMARKERS OF RADIATION EXPOSURE IN THE ENVIRONMENT | 277 |
123 CYTOGENETIC ASSAYS | 280 |
124 USING CYTOGENETIC BIOMARKERS FOR ENVIRONMENTAL BIODOSIMETRY | 286 |
ECOTOXICOLOGICAL CHARACTERIZATION OF COMPLEX MIXTURES | 293 |
133 PRINCIPAL ENVIRONMENTAL SAMPLES USED FOR ECOTOXICOLOGICAL EVALUATION | 298 |
134 GLOBAL ECOTOXICITY EVALUATION | 300 |
135 NONFISH CELL CULTURES | 302 |
136 FISH CELL CULTURES | 307 |
32 INTRODUCTION | 18 |
33 METHODOLOGY | 21 |
34 CELL POPULATIONBASED IN VITRO APPROACHES TO NORMAL TISSUE TOXICOLOGY | 23 |
35 VALIDATION OF DATA FROM PRIMARY EPITHELIAL CULTURE SYSTEMS | 28 |
36 CONCLUSIONS | 29 |
CULTURED EPITHELIA FROM FISH GILLS | 35 |
42 GILL CELLS IN MONOLAYER CULTURES | 36 |
43 CULTURED GILL EPITHELIA | 37 |
44 CONCLUDING REMARKS | 48 |
END POINT AND TOOLS FOR IN VITRO TOXICOLOGY | 53 |
APPLICATION OF IN VITRO TECHNIQUES IN STUDIES OF BIOMARKERS AND ECOTOXICOLOGY | 55 |
52 DETOXIFICATION SYSTEMS OF INVERTEBRATES AND AQUATIC VERTEBRATES AS BIOINDICATORS OF ENVIRONMENTAL POLL... | 60 |
53 PRIMARY CELL CULTURES | 61 |
54 USES OF CELL LINES IN TOXICOLOGY | 65 |
55 NONMAMMALIAN TISSUE CULTURE | 68 |
56 POTENTIAL OF LOWER VERTEBRATE AND INVERTEBRATE TISSUE CULTURE IN TOXICOLOGY | 69 |
END POINTS FOR IN VITRO TOXICITY TESTING WITH FISH CELLS | 77 |
62 CELL SYSTEMS FROM TELEOST FISH | 78 |
63 END POINTS IN IN VITRO ASSAYS | 82 |
64 PERSPECTIVES | 124 |
TOXICOGENOMIC TECHNOLOGIES FOR IN VITRO AQUATIC TOXICOLOGY | 143 |
72 TOXICOGENOMICS | 144 |
74 MICROARRAYS | 149 |
75 PROTEOMICS | 152 |
76 CONCLUSIONS | 155 |
DELAYED AND INDIRECT GENOTOXICITY A PARADIGM SHIFT IN TOXICOLOGY? | 161 |
82 THE BIOLOGICAL EFFECTS OF IONIZING RADIATION | 162 |
83 RADIATIONINDUCED BYSTANDER EFFECTS | 169 |
84 A LINK BETWEEN RADIATIONINDUCED GENOMIC INSTABILITY AND BYSTANDER EFFECTS | 174 |
85 CONCLUSIONS | 175 |
SPECIFIC TOXINS AND TOXIC GROUPS | 183 |
TOXICOGENOMIC APPLICATIONS IN ENVIRONMENTAL TOXICOLOGY | 185 |
92 METHODOLOGICAL APPROACHES FOR DIFFERENTIAL GENE EXPRESSION | 187 |
93 RESTRICTION ENDONUCLEASEFACILITATED ANALYSIS OF GENE EXPRESSION | 188 |
94 SUBTRACTIVE HYBRIDIZATION SH METHODS | 189 |
METHODS AND TOXICOLOGICAL APPLICATIONS | 194 |
96 EVALUATION | 213 |
BIOMARKERS TO ASSESS ENDOCRINE DISRUPTION OF REPRODUCTION IN BIVALVES | 221 |
102 HORMONAL REGULATION OF REPRODUCTION | 223 |
103 BIOMARKERS TO ASSESS ENDOCRINE EFFECTS ON REPRODUCTION | 225 |
104 CASE STUDIES | 233 |
105 GENERAL CONCLUSIONS | 235 |
FISH CELLS USED TO DETECT AQUATIC CARCINOGENS AND GENOTOXIC AGENTS | 239 |
112 IN VITRO ASSAYS FOR THE EVALUATION OF MUTAGENICITY CARCINOGENICITY OF CHEMICALS | 241 |
113 FISH CELLS IN THE GENETIC ASSESSMENT OF CONTAMINANTS TO WILD POPULATIONS OF AQUATIC ORGANISMS | 255 |
114 CONCLUDING REMARKS | 262 |
137 TOXICITY IDENTIFICATION AND REDUCTION EVALUATION PROCEDURES | 313 |
138 CONCLUSIONS AND LIKELY FUTURE TRENDS | 317 |
IN VITRO ECOTOXICOLOGICAL ASSESSMENT OF AQUATIC SEDIMENTS | 325 |
142 ASSESSING THE CHARACTERISTICS OF AQUATIC SEDIMENTS | 327 |
143 SEDIMENT COLLECTION STORAGE AND PREPARATION GENERAL CONSIDERATIONS | 329 |
144 SEDIMENT PREPARATION METHODS | 332 |
145 IN VITRO TESTS FOR THE TOXICOLOGICAL ASSESSMENT OF SEDIMENTS | 345 |
146 USE OF MICROSCALE TESTS FOR ASSESSING SEDIMENT TOXICITY | 347 |
147 USE OF IN VITRO CELL CULTURES IN DETERMINING SEDIMENT TOXICITY | 351 |
148 CONCLUSIONS | 356 |
149 PERSPECTIVES AND FUTURE RESEARCH | 357 |
PRACTICAL ISSUES | 373 |
IN VITROIN VIVO BRIDGING APPROACHESVALIDATING THE RELEVANCE OF IN VITRO TECHNIQUES WITH REFERENCES TO THE W... | 375 |
153 INHERENT ADVANTAGES OF IN VITRO AQUATIC TOXICOLOGY RESEARCH MODELS | 377 |
154 INHERENT PROBLEMS WITH IN VITRO RESPONSES IN AQUATIC TOXICOLOGICAL STUDIES | 378 |
155 AQUATIC TOXICOLOGYIN VITROIN VIVO RESPONSES | 379 |
156 CELL LINES VERSUS PRIMARY CULTURE | 380 |
157 REDUCTION AND REPLACEMENT OF IN VIVO TESTS | 381 |
158 BRIDGING THE IN VIVOIN VITRO DIVIDE | 386 |
159 WHY PRIMARY CELL CULTURES? | 388 |
1510 IN VITROIN VIVO END POINTS AND RESPONSES | 389 |
1512 CONCLUSIONS | 390 |
397 | |
398 | |
163 HAEMOPOIETIC CELL CULTURES | 401 |
164 LYMPHOCYTE CULTURES | 402 |
165 PHAGOCYTE CULTURES | 405 |
166 CONCLUSIONS | 411 |
419 | |
172 THE ECVAM VALIDATION PROCESS | 421 |
173 THE ICCVAM TEST METHOD EVALUATION PROCESS | 425 |
174 REGULATORY TESTS | 427 |
175 CONCLUSIONS | 429 |
433 | |
184 THE NUREMBERG CODE AND ITS IMPACT ON ANIMAL TOXICOLOGY | 435 |
THE ANTHROPOCENTRIC VIEW IS MADE LEGAL | 436 |
187 THE PROBLEM OF ASSESSMENT OF VALUES BY ONE GROUP OR SPECIES ON BEHALF OF ANOTHER | 437 |
188 DEEP ECOLOGY AND THE BIOCENTRIC VIEWPOINT APPLIED TO TOXICOLOGY | 438 |
189 ALTRUISM TOWARD ANIMALS IN RESEARCH | 439 |
THE MATHEMATICS OF CELL GROWTH IN CULTURE | 441 |
442 | |
194 SIGNIFICANCE OF CELL GROWTH MODELLING | 444 |
195 LINKING ORGANIZATIONAL LEVELS | 450 |
196 CONCLUSIONS | 455 |
CONCLUSIONS | 457 |
Términos y frases comunes
activity analysis animal Aquatic Toxicology assessment bioassays Biochemistry Biology biomarkers Blaise Braunbeck bystander effect carcinogens cDNA cell cultures cell lines cellular chemical comet assay compounds concentration contaminants CYP1A cytochrome cytotoxicity cytotoxicity tests detection developed differential ecotoxicology effluents end points endocrine environment Environmental Toxicology enzyme epithelial cells estrogenic evaluation experimental exposed exposure fibroblasts fish cell lines fluorescence freshwater function Gagné gene expression genetic genotoxicity gill gonad hepatocytes human hybridization identify induced invertebrates irradiated isolated hepatocytes Journal laboratory levels liver lymphocytes mammalian cells marine measured membrane metabolic methods microarrays micronucleus Molecular Mothersill mRNA mutagenic Mutation Research neutral red Oncorhynchus mykiss organisms Pärt pollutants population pore water potential primary cell primary cultures protein radiation rainbow trout rainbow trout hepatocytes receptor response samples sediment sediment extracts sediment toxicity Segner sensitive species studies techniques tissue toxicity toxicity testing toxicogenomics Toxicology and Chemistry vitellogenin vivo xenobiotics